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Work fast with our official CLI. Learn more. If nothing happens, download GitHub Desktop and try again. If nothing happens, download Xcode and try again. There was a problem preparing your codespace, please try again. Please read the Contributing Guidelines before contributing.

You can find some interesting performance timing comparisons of various frameworks in the following locations. Alf is an out of this world super fast and configurable framework for ZSH; it’s modeled after Prezto and Antigen while utilizing Oh-My-Zsh under the covers; and offers standard defaults, aliases, functions, auto completion, automated updates and installable prompt themes and plugins.

It uses antigen to manage bundles and oh-my-zsh. Can load bundles conditionally. By default it includes the powerlevel9k theme, autosuggestions, syntax-highlighting and fzf-widgets and fzf-marks. Fully customizable. Ant-zsh is a tiny and lightweight ZSH configuration environment for special customization needs. It includes plugins, themes and a basic convenient setup. Antibody is a faster and simpler antigen written in Golang.

Antidote is a ZSH plugin manager made from the ground up thinking about performance. It is fast because it can do things concurrently, and generates an ultra-fast static plugin file that you can include in your ZSH config.

It is written natively in ZSH, is well tested, and picks up where Antibody left off. It will automatically clone plugins for you. Antigen is a small set of functions that help you easily manage your shell ZSH plugins, called bundles.

Antigen is to ZSH, what Vundle is to vim. Antigen can load oh-my-zsh themes and plugins and will automatically clone them for you. It provides sane defaults and is extendable by plugins.

Ax-ZSH integrates well with Powerlevel10k and other extensions, including plugins compatible with oh-my-zsh. Dotzsh strives to be platform and version independent. Some functionality may be lost when running under older versions of ZSH, but it should degrade cleanly and allow you to use the same setup on multiple machines of differing OSes without problems. We also support files such as ackrc and gitconfig. Think of it as Bundler for your dot files.

PMS allows you to manage your shell in a way to that helps decrease setup time and increases your productivity. It has support for themes change the way your shell looks , plugins adds functionality to your shell , and dotfile management. The PMS framework also allows you to use the same framework in different shells. Use ZSH on your personal laptop, and use bash on remote servers.

Wanna try fish? Go ahead, try out different shells. Prezto enriches the ZSH command line interface environment with sane defaults, aliases, functions, auto completion, and prompt themes. An easy solution for managing your shell environments. Works with both bash and zsh. Makes it easy to sync your environment across multiple machines with a git repository. Toasty is a ZSH framework made to facilitate management, not dictate it.

Yazt is a simple ZSH theme manager in maintence that is compatible with nearly everything. You can use prompts in plugins, mix ‘n’ match two themes, with a few modifications, you can even use it in bash. In addition to loading and updating plugins stored in git repositories, it supports lazy-loading plugins further reducing startup time as well as downloading and sourcing code snippets.

Zeesh is a cross-platform ZSH framework. It’s similar to, but incompatible with, oh-my-zsh. It has a modular plugin architecture making it easy to extend. It has a rich set of defaults, but is designed to be as lightweight as possible. Zgen is a lightweight plugin manager for ZSH inspired by Antigen. The goal is to have minimal overhead when starting up the shell because nobody likes waiting.

It is currently not being actively maintained and I recommend you use the zgenom fork instead, which is actively maintained and extended. A lightweight plugin manager for ZSH that is a fork that extends the brilliant zgen and provides more features and bugfixes while being fully backwards compatible. To keep loading fast during new terminal sessions, zgenom generates a static init. This minimizes startup time by not having to execute time consuming logic plugin checking, updates, etc during every shell session’s startup.

The downside is that you have to refresh the init script manually with zgenom reset whenever you update your plugin list in your. Zgenom can load oh-my-zsh -compatible and prezto -compatible plugins and themes, and will automagically git clone plugins for you when you add them to your plugin list. Formerly known as zplugin and zinit. ZI – Wiki Pages. Bonus: you can use zi-console to view and change the state of the ZSH session e. Zim is a ZSH configuration framework with blazing speed and modular extensions.

Zinit is an innovative and probably because of the Turbo the fastest plugin manager with support for:. It is minimal because it implements the bare minimum to be qualified as a plugin manager: it allows the user to install plugins from git repositories and git repositories only, that’s why the name , source plugins and update them. While tailored for ZSH plugins specifically, Znap also functions as a general-pupose utility for managing git repositories.

Zoppo is the crippled configuration framework for ZSH. As an Italian saying goes: “chi va con lo zoppo, impara a zoppicare”, we realized we were walking with a cripple and are now going to become crippled ourselves. The minuscule ZSH package manager.

At first run, zpm will do complex logic and generate a cache, after that will only use the cache, so it makes this framework very fast. Ztanesh aims to improve your UNIX command line experience and productivity with the the configuration provided by the ztanesh project: the tools will make your shell more powerful and easier to use. A simple and fast ZSH plugin manager.

It uses zcompile and async loading to speed up your shell startup time. Zulu is a environment manager for ZSH 5 or later, which aims to make it easy to manage your shell without writing any code.

A full terminal configuration framework including zsh , tmux , fzf , vim and spacemacs configurations. It includes:. If you’re using zgenom , add them to your init. Antigen will handle cloning the plugin for you automatically the next time you start zsh. Zgen is not being actively maintained.

Consider switching to the Zgenom fork, which is. Zgenom will automatically clone the plugin repositories for you when you do a zgenom save. I’ve documented some recommendations for writing new plugin and themes here. There is also a more detailed Zsh Plugin Standard.

Mastering ZSH is a great tutorial that builds on the basics to show you advanced ZSH usage, customizations, and practical examples. Skip to content. Star A collection of ZSH frameworks, plugins, themes and tutorials. License BSDClause license. This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.

Branches Tags. Could not load branches. Could not load tags. Launching Xcode If nothing happens, download Xcode and try again. Launching Visual Studio Code Your codespace will open once ready. Latest commit. Git stats 4, commits. Failed to load latest commit information. Update issue template. Jul 20, Nov 19, Feb 15, Update Code of Conduct. Jun 21, Update contributing guidelines.


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Supports Elementary OS Freya. Doesn’t touch your existing. Includes pipenv completions. Very oh-my-zsh centric, the generated plugins will need editing to work with other frameworks. Includes tab-completions. It stores the current rate to a file in the RAM backed filesystem where it can be easily accessed for display in a shell-prompt. It also increases the terminal window’s contrast to enhance visibility.

Some generated themes require powerline-compatible fonts. Installs, updates or loads pyenv , and adds extra functionality. Also loads pyenv-virtualenv if available. Designed for use with dotpyvenv. Requires OpenRazer linux drivers. This plugin will install rbenv and make it readily available rbenv elliottcable – A faster fork of the rbenv plugin from oh-my-zsh. Depends on ripgrep. See Conrad Irwin’s bracketed-paste blog post. When risky patterns is detected you will immediately get a small prompt challenge that will verify your action.

As it makes use of the module command it is much more efficient than spack load. It automatically registers fonts and sets all necessary environment variables to run the t32 toolset.

Forked from termsupport. Adds workspace when not default to prompt. Also installs terraform-docs , tfsec and tflint. Includes a function to manually update tgenv. Better with dark terminal themes. An example use case is to hold logs in files This makes it very easy to go up a few directories without having to type any commands. Only works with the oh-my-zsh framework. Useful for checking URL typos.

The plugin also ships with a few extra commands that help to manage more than one box, along with custom tab-completion. Adds support for customizing the virtualenv prompt in oh-my-zsh themes.

Has per project stats. Because cd seems inefficient when the folder is frequently visited or has a long path. This packages the instructions from wsl2-ssh-pageant repo as a ZSH plugin. An alternative to z. Requires tmux.

See the github page for installation details. Includes git and hg status decorators. Useful for debugging. Supports all data types. It is similar to selecta , but uses the curses library to do display, and when compared to fzf , the main difference is approximate matching instead of fuzzy matching. Completions These plugins add tab completions without adding extra functions or aliases.

Note – this does not automatically generate completions as needed, you have to explicitly run it to generate completions for a command. It also contains completions and combines support for fzf. It’s meant to improve the usual workflow build unittest benchmark Works with npm and yarn. Only works with Ubuntu Includes a single git marker for VS Code. Works well on dark backgrounds by default but colors can be easily customized.

Also includes decorators for AWS profile name, virtual environment name if any, number of background tasks, current directory and previous command’s exit code if non-zero. Shows detailed information about git status. Very customizable. Requires a unicode font and works best with a solarized terminal. Works better with a unicode font. Includes decorators for status of last command run, user hostname, git status, working directory, whether running as root, whether background jobs are running, and other information.

Includes git status. Includes git and mercurial status, current time and time the last command took decorations in the prompt. Requires a Powerline-compatible font. Includes git , virtualenv and node status decorations.

Faster than many other prompts because it determines the git decorations asynchronously in a background process. Includes git status, user host, last command exit status and working directory decorations alpharized – Optimized to work with solarized dark terminals. It’s a modified version of the avit theme. Displays only the necessary information: current user, hostname, working directory, git branch if one exists.

Looks great both with dark and white terminals. Colors customizable by setting vars in your. Includes git status decorations. Based on the norm theme. Includes path and git decorations. Works well with the zsh-users zsh-syntax-highlighting plugin.

Reminiscent of powerline. Requires Crystal to build. Includes decorations for kubernetes context, current directory, last command exit status and git status. Includes git , rvm and virtualenv status decorations.

Designed to be used with the bliss iTerm color scheme and bliss dircolors. Requires a font with unicode support. It consists of blocks: each block is shown inside a pair of [square brackets], and you can add blocks by simply creating a function. Includes git and ruby version decorations. Includes decorators for username hostname , current directory, git status, return code for last command, and time last command was run.

It includes decorators to display git status asynchronously, a notification if the ssh session is remote, the battery level and the number of background jobs. Includes decorations for username, host, current directory, and git status. Requires nerd-fonts. Works best with nerdfonts. Changes color when root. It aims for simplicity, showing information only when it’s relevant. Includes git decorations. Includes git status optimized for large repositories. Based loosely on Fox and Jonathan themes from oh-my-zsh.

Includes git status decorations, Kubernetes cluster information if any , current AWS profile and region, and active virtualenv. Depends on autojump , zsh-autosuggestions and zsh-syntax-highlighting.

Requires powerline-compatible fonts and the git-prompt plugin. Requires a powerline-compatible terminal font. Works better with dark backgrounds. Pure is not clean, clean is not pure.

Works with standard fonts. Includes node and git status decorations. Does not require special fonts. Has git and last command exit status decorations. Requires a nerdfont to render properly. Includes an iTerm color settings file. Requires nerdfonts. Looks similar to powerline. Has customizable segments, decorators for git status, rust , node. Includes git status and working directory decorations. Includes directory, git status, and hostname decorations.

Works best with a unicode font. Includes git status decorations, username and hostname abbreviations, virtual environment, current working directory, return status of last command and the time.

Includes git status decorations and a clock. Requires powerline-compatible font. Includes git and virtualenv status decorations.

Includes git status indicator, vi -mode indicator, current directory and current path. Includes a battery gauge and git status display that can be enabled on the right-hand side of the prompt. Frank keeps to the facts and only tells you extra things when they are important. Includes git status integration. Includes many different status cars. Inspired by the gitsome theme. In particular the branch name, difference with remote branch, number of files staged or changed, etc.

Shows decorators for truncated current directory, hostname and git status. Includes both light and dark modes and detects system settings for that on macOS and Linux.

Uncludes git status, user host, path and date decorators. Includes git status, current directory, root status decorations. Includes decorators for git status, time and current directory.

Related to powerlevel9k. This theme was inspired by a keycap set called DSA Hana. This pairs well with the hana atom theme. Shows root status, git status, current directory and user hostname decorations. It shows username, hostname, current directory and git status decorations. Works with macOS out-of the box, includes git status decorations. Includes decorations for the current directory and git status. Includes git status information. Includes decorators for git status and the current directory.

Includes decorators for git status, username and current directory. Includes user host, git status and time decorators. Based on the obraun theme. Includes an optional iterm2 color scheme. Includes decorators for git status, whether running as non-default user and current working directory. Includes current working directory and git status decorations. Requires Powerline-compatible terminal font.

Includes decorators for the exit code of the last command run, python virtualenv and VCS status. Includes prompt decorations for git status, last command exit status, user hostname, working directory and whether the user is running as root.

Includes decorations for git status, current directory and the kubectl context and namespace. Has time, directory, and git status decorations.

The primary color is blue with a magenta accent. Requires Nerd Fonts and the gitstatus plugin. Shows root status and git status decorations.

Includes git status and background job decorations. Includes git status and ruby version decorations. Includes git status, time, username host, working directory and last command exit status decorations. Shows git status information. Includes segments with git status, Pythonvirtualenv, current directory and current time.

Includes git status and last command exit decorations. Includes decorations for root status, current directory, git status, current ruby rvm environment and current python virtualenv. It shows you what you need when you need it. You will notice what changes when it changes, saving time and frustration.

No roughness, just smooth. Includes git , user host, last command exit status, current directory, current time and root status decorators. Shows remote status and indicator for vi-mode. Includes git status, works with standard fonts. Has git decorations, requires a powerline-compatible terminal font. Based on the frisk oh-my-zsh theme.

Includes decorations for git status and the status of the last command run. Includes status codes for active python venv , exit status of last command, shortened working directory, git status decorations. Designed to be easy on the eyes. Based on the kphoen and smt themes.

Requires the hooks plugin. Reads the battery percentage grepping acpi command and displays it in the prompt. On the widget editor view, use the Rate drop-down menu to select rate measurement 10, then click the back arrow upper-left corner of the widget editor view to return to the analysis view.

In addition to the Energy Map widget described below, there are several additional scatter plot widget options you can add to your analysis views that are assay type dependent and defined in the Analysis Views section. Energetic Map Basal. Please also note that buffer factor must be properly assigned to the assay media, background wells, and assay groups to calculate this widget data.

Calculations used for this widget can be found in the details on the Add Widget dialog pictured right. For XF ATP Rate assays with more than 2 injections, you must identify the oligomycin injection using the drop-down menu seen on the Add Widget pictured right before you can add this widget to your analysis view. This oligomycin injection selection plays a critical role in calculating the correct Basal ATP production rates that you will see on the widget.

Incorrect selection of the oligomycin injection will result in incorrect calculations and data. Energetic Map Induced. Please also note that buffer factor must be properly assigned to the assay media, background wells, and assay groups before you can calculate this widget data. Calculations used for this widget can be found in the details on the Add Widget dialog pictured below, right. The oligomycin injection selection plays a critical role in calculating the correct induced ATP production rates that you will see on the widget.

XF Cell Energy Phenotype. This is a unique widget compared to the other scatter plot widget options in Seahorse Analytics as it plots 2 data points per group — the baseline phenotype and the stressed phenotype, connected by a dashed line called the metabolic potential — and the y-axis is always OCR and the x-axis is always ECAR pictured below.

This widget applies to both the standard and induced assay workflows. The ATP Production Rate Induced widget creates a stacked bar chart of the average of the induced glycoATP production rates upper blue bar and the average of the induced mitoATP production rates bottom red bar for each group pictured below, right.

This widget applies to the induced assay workflow only. The XF ATP Rate Index widget plots the ratio of basal mitoATP production rate to basal glycoATP production rate as an empty circle marker, and plots the ratio of the average of the induced mitoATP production rates to the average of the induced glycoATP production rates for each group as a filled circle marker only when applied to an induced assay result file — widget pictured below shows data from an induced XF ATP rate assay. Export your data to a Microsoft Excel or GraphPad Prism file or share your result data and insight with a collaborator directly using Seahorse Analytics.

You can create an Excel and Prism file containing all rate data for the selected file. This export will not include any parameter calculations i. Spare Respiratory Capacity that you have calculated in an analysis view, you will need to export that data individually from each widget. How is this export feature used? This type of data export allows users to easily transfer all rate data for OCR, ECAR, and PER data into Prism or Excel to create high-quality or customized figures, perform statistical analysis, and other analytical functions not offered in Seahorse Analytics.

Export select data from a widget. You can export individual widget data to an Excel and Prism file containing data for the selected widget. The data in the export file will match exactly how you formatted the widget.

For example, if you configure a widget to display basal respiration in well mode, the Prism export file will contain individual well values for basal respiration data for each group. If you configure a widget to display basal respiration in group mode, the Prism export file will show the average group value and error value, not individual well values. This type of data export allows users to easily export select rate or parameter data as an image file, or as a Prism or Excel file.

The Prism and Excel export allow you to get exactly the data you want to further analyze i. You can share individual assay result files with collaborators or your team using the built-in file share feature. The share feature is found under the small 3-dot menu to the right of each assay result file on the Home and Files views pictured right. If the recipient does not have a Seahorse Analytics account, they must create one to view the data file. If the recipient has an account, they will receive an email notifying them a file was shared with them.

They will also see a notification in Seahorse Analytics bell icon in the upper-right corner where they will accept or reject the shared file. How is this sharing feature used? The share feature allows you to send an assay result file to someone with, or without, a Seahorse Analytics account directly through the application.

The recipient can make modifications to the shared file, but your copy of the file will remain unchanged. Transform complex cellular metabolism data into publishable results using Wave Desktop’s flexible analysis views, embedded reporting tools, and other powerful analytical capabilities. It is always encouraged to update to the latest version of Wave Desktop software. The latest release of Wave Desktop software is version 2. Below you will find the PC specs and compatibility detail for Wave Desktop 2.

Cellular oxygen consumption respiration and proton excretion glycolysis cause rapid, easily measurable changes to the concentrations of dissolved oxygen and free protons. Only available in post-run assay results, not during assay runtime. See chapter 3 in the Wave User Guide for more information about the types of data available in Wave software.

Oxygen Concentration mmHg : As cells or other biological material consumes oxygen during a measurement, oxygen tension will decrease. This decrease in oxygen tension is used to calculate the rate of oxygen consumption OCR. You can view oxygen tension level data on the Overview analysis view using the Y1 drop-down menu. Oxygen tension O 2 data displayed as mmHg vs.

Proton Concentration mpH : As cells or other biological material generate protons during a measurement, proton concentration will increase. The concentration of free protons in the microchamber is measured throughout an XF assay and calculated as the rate of extracellular acidification ECAR. You can view mpH level data on the Overview analysis view using the Y1 drop-down menu. Proton Concentration mpH data displayed in Level mode right. Depending on the type of analysis view selected, Wave automatically calculates and graphs result data as one of the following:.

A kinetic graph displays the rate on the y-axis, and time on the x-axis. During an assay, data is acquired and plotted in real-time as a kinetic graph. Kinetic graphs can be found in the Quick View and Overview analysis views in Wave software. Use the Measurement drop-down menu to select a rate measurement to display on the energy map for each group.

Use the Rate drop-down menu to change the x-axis rate to PER. ECAR analysis views. The Plate Map displays rate data for the selected rate measurement of each assay well. The Quick View has a button to display the Plate Map, which is hidden by default. When opening a new analysis view, the Plate Map shows data for rate measurement 1 by default.

Use the Rate drop-down menu to display data for another rate measurement during the assay. The Bar Graph is available on the Overview analysis view only below the Plate Map , and displays the average rate for each group for the selected measurement.

Use the Display drop-down menu to change the rate display from Group average to Well individual well mode. The Bar Graph can be used for identifying outliers, optimal FCCP concentration, optimal cell seeding density, or other trends that may not be apparent when measurement data is plotted as a kinetic graph or scatter plot. The Group List is the legend for the data plotted in the kinetic graph or scatter plot.

There are 4 customizable analysis views that can be added to an assay result file in Wave Desktop. Each analysis view can be added to an assay result file multiple times using the Add View button in the top-level ribbon menu.

Quick View is the default analysis view displayed when opening a new assay result file. The selected rate is displayed on the y-axis, and time is displayed on the x-axis. Group statistics average rate and error for each measurement can be displayed by checking the Details box in the Group List below the kinetic graph.

Overview is the most versatile analysis view in Wave software for routine analysis functions. To display Overview , click Add View and select Overview from the list of views.

This gives greater flexibility for tailoring your result data presentation to show specific groups, responses, or comparisons between groups. The OCR vs. OCR is always displayed on the y-axis, and cannot be changed. To display OCR vs. ECAR from the list of views. The Data view contains all data associated with the assay result file organized into 7 tabs:.

To display the Data view, click Add View and select Data from the list of views. The Group Data tab is displayed by default when opening the Data view. Click the Export button in the top-level ribbon menu to display the list of available XF Report Generator export options. This section of the XF Learning Center will provide an introduction to various topics on using XF Analyzers, including a range XF assay kits and applications, alternative assay conditions and sample types, as well as normalization and analysis of XF data.

Agilent XF Assay Kits and reagents are developed specifically for use with each XF Analyzer to ensure reliability and consistency of results. Seahorse XF kits and reagents help simplify running an XF assay by providing pre-calibrated, pre-tested reagents for measuring valuable functional metabolic parameters including cellular ATP production rates, mitochondrial function, glycolytic activity and substrate oxidation in living cells, permeabilized cells and isolated mitochondria.

The materials below provide information and methods for performing a wide range XF Assays. Normalization of XF assays can be applied on several levels, including cell number, genomic DNA, and total cellular protein.

The materials below provide information on methods for normalization of XF Data for your. The regulation of metabolic pathways and energy expenditure, and how differential substrate oxidation contributes to metabolically regulated signaling mechanisms is an important topic for researchers in divers fields such as immunology, cancer and stem cell biology and drug target identification and mechanism-of-action studies.

The study of mitochondrial function is central to both clinical and basic scientific research. However, examining the precise enzyme or pathway driving observed changes can provide additional insight and further link-specific alterations in metabolic enzymes with disease states.

A traditional method of studying substrate oxidation involves isolating mitochondria, and the XF Analyzers support a high-throughput assay in which both energy demand and substrate availability can be tightly controlled for mechanistic studies using minimal quantitiues of isolated mitochondria. However, there are several disadvantages when isolating mitochondria, including limited quantity and a bias stemming from the sub-selection or damage of mitochondria during isolation.

XF Plasma Membrane Permeabilizer PMP forms pores in the plasma membrane of adherent cells without causing any concomitant damage to the mitochondrial membrane. This reagent overcomes the challenges associated with using isolated mitochondria or substrate-supplemented media with intact cells.

Analyzers have the capability of measuring metabolism in reduced oxygen environments hypoxia , as well as with certain types of three-dimensional samples, including islets. Analyzers have the capability of measuring metabolism in reduced oxygen environments hypoxia , as well as with certain types of three-dimensional samples, including spheroids.

Analyzers have the capability of measuring metabolism in reduced oxygen environments hypoxia. Shop the online store to purchase cartridges, plates, kits, media, and reagents to your your XF assay. Gather materials 1. Prepare for assay 2. Set up assay 3. Run assay 5. Analyze results W 5. Beyond the basics 6. Showing only information for the Seahorse Change instrument type.

Select a workflow step to display help content. Basic procedures for choosing cell seeding densities To effectively examine metabolic and bioenergetic function using your Agilent Seahorse Extracellular Flux Analyzer, it is essential to first characterize a specific cell type with respect to its metabolic activity under basal and maximal respiration OCR and extracellular acidification ECAR.

Experiment Rationale Accelerated Workflow Standard Workflow Seed cells at single or different densities and visually assess degree of cell confluence; choose a miniplate for the next step. Visual assessment is a good first approximation of optimal cell density and will be verified in each assay.

Seed miniplates at different densities according to the diagram below. Seed 1 miniplate at a single cell density; hydrate 1 XFp cartridge. Choose cell densities to test, based on standard or accelerated workflow described above. Remove a three-pack of miniplates from the blue box. Remove the foil seal from the tub s that will be used.

Add sterile water or PBS to the moat around the cell culture wells. These are background correction wells. Within the Cell Type group definition, click Cell Type and Add to add a new cell type entry and enter the name of cell type you intend to analyze in your assay.

It is recommended to add the seeding density to group name. When designing your assay template, you can: Create a new assay template for the 3rd and 4th cell seeding density groups.

Add 4 cell seeding density groups to one assay template and reassign the 3rd and 4th cell group to the plate map after performing the first assay with cell seeding density groups 1 and 2. Rename groups in this template after performing the first assay with cell seeding density groups 1 and 2. Assign the first cell seeding density group to the plate map.

To assign a group to the plate map, first click the group name in the group list, then: Click the column header i. A, B, C, etc. Click individual wells on the plate map. Repeat for the next cell seeding density group. Modifying the instrument protocol settings directly affects how data is acquired during your assay. For this example, the default instrument protocol is used and recommended. Should you need to modify the default instrument protocol, prior to performing your cell seeding density optimization assay it is recommended to review the instrument protocol section in the Wave User Guide , or if necessary, contact Agilent Cell Analysis Technical Support.

Place the sensor cartridge upside down next to the utility plate. Remove and discard the water from the utility plate. Lower the sensor cartridge onto the utility plate, submerging the sensors in calibrant. Remove the assembled sensor cartridge with Hydro Booster and Utility plate from the incubator. Remove and discard the Hydro Booster. A: image shows low end of optimal cell density, ideal seeding density and distribution.

B: image shows high end of optimal seeding density, some cell clusters can be seen. Place row labels lettered A-H to the left. The triangular notch will be in the bottom left-hand corner.

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Connect an appropriate interface between the computer and the car. The standard license allows you to register up to 5 bikes. Select “software update”.


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